SACE0L03674g

3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs

Genomic environment map

Element type: CDS
Element length: 1044 nucleotides,
on sense strand of
Sace0L: 341811..342854.
Other names:
ERG27
YLR100W
Coding sequence: 348 codons.

Database cross references:
CYGD: YLR100w
EMBL: AAB67544.1
EMBL: CAA97664.1
EMBL: U53876
EMBL: Z73272
Ensembl: YLR100W
GeneID: 850790
GenomeReviews: Y13138_GR
HOGENOM: Q12452
NMPDR: fig|4932.3.peg.4193

Computed results

None available yet

Homologs and Orthologs

Génolevures family GL3R2390
Robust family of 9 members, scrltkgdy (1 1 1 1 1 1 1 1 1)
9-member family, phyletic pattern sczltkgdy, profile (1 1 1 1 1 1 1 1 1)
Proteins: SACE0L03674p CAGL0M11506p ZYRO0D06534p SAKL0H16368p KLTH0G13046p KLLA0F19756p ERGO0G09878p DEHA2E20130p YALI0B17644p
Search in: Génolevures Google

Génolevures family GL3R2390.N1
Nine-SONS subgroup of a Robust family
9-member family, phyletic pattern sczltkgdy, profile (1 1 1 1 1 1 1 1 1)
Proteins: SACE0L03674p CAGL0M11506p ZYRO0D06534p SAKL0H16368p KLTH0G13046p KLLA0F19756p ERGO0G09878p DEHA2E20130p YALI0B17644p
Search in: Génolevures Google

Homologs in protein families: GL3R2390 GL3R2390.N1
Orthologs: strict determination not possible; homologs must be refined manually

Protein SACE0L03674p

Protein domain map

Protein length: 347 amino acids
Protein family: GL3R2390

Database cross references:
DIP: DIP:1237N
Gene3D: G3DSA:3.40.50.720
GermOnline: YLR100W
IntAct: Q12452
InterPro: IPR002198
InterPro: IPR016040
KEGG: sce:YLR100W
LinkHub: Q12452
PANTHER: PTHR19410
PIR: S64936
PeptideAtlas: Q12452
RefSeq: NP_013201.1
SGD: S000004090
UniProtKB/Swiss-Prot: Q12452
UniprotKB: ERG27_YEAST

Computed results for SACE0L03674p

Blastp Genolevures

Blastp Uniprot

Gene Ontology terms

GO:0006696	ergosterol biosynthetic process
GO:0005789	endoplasmic reticulum membrane
GO:0005741	mitochondrial outer membrane
GO:0005515	protein binding
GO:0000253	3-keto sterol reductase activity

Sequence data

>SACE0L03674g.nt
CTATCCATAATTTGCTGCTGCAGGGCCTTCAGGCCCTGCACGCTAGCACACACCTTGGCG
TCTGCAGTAGTGGTCTCCTTCTCAGTTTTGCTGCACCATTTGAAAGTGTTTGTCCACATT
CTACTGAAGAAAACTACGCTTACGTATGTATATGTCCTGAACAATGAGAGAAAGACCTGC
GAATATACTTCTACTACTCCACGTACAAAAAAGAGCACGCTGCTTTATTTATACTTTTGT
GCCACAAGAATGATCAACATCAACATAAATATCAACTAGTATCTGCAACACATCTGCTCC
ACGGAACTAAACCCGTTGAGCAGTGCCCCGTGGAAACGTAAACTATCGCAAATTGGGATT
AACAAGCCAAAAACAGCCAAGCAAGATTCACGAAACCGCGCCTCGTTTGGACCCCGAAGG
CCCATTTAACGGCCGGCCGTTACAAGCAAGATCGGCAGAGCAAACCACTCCCCAGCACCA
CAGCACATCACTGCACGAGCAACAATAACTAGAACATGGCAGATAGCGAGGATACCTCTG
TGATCCTGCAGGGCATCGACACAATCAACAGCGTGGAGGGCCTGGAAGAAGATGGTTACC
TCAGCGACGAGGACACGTCACTCAGCAACGAGCTCGCAGATGCACAGCGTCAATGGGAAG
AGTCGCTGCAACAGTTGAACAAGCTGCTCAACTGGGTCCTGCTGCCCCTGCTGGGCAAGT
ATATAGGTAGGAGAATGGCCAAGACTCTATGGAGTAGGTTCATTGAACACTTTGTATAAG
TGTTTGTTGTTTATGTATCCGCATATAGCAGTTATAACAGATAAATGGCACTTTTCGCAC
ACCCGTTGTTTTATCTCCGATAGTACGTGGGCCTTTATTTATGGTCGTTTAACGAAAGAA
CGGCATCTTGAATTGAGCAGGTATTTAAAAGATAGGACGAGAAACAAGCACATGATCTGT
GTCGAAAAAAAGTAGCAAAGAGAAAAAGTAGGAGGATAGGATGAACAGGAAAGTAGCTAT
CGTAACGGGTACTAATAGTAATCTTGGTCTGAACATTGTGTTCCGTCTGATTGAAACTGA
GGACACCAATGTCAGATTGACCATTGTGGTGACTTCTAGAACGCTTCCTCGAGTGCAGGA
GGTGATTAACCAGATTAAAGATTTTTACAACAAATCAGGCCGTGTAGAGGATTTGGAAAT
AGACTTTGATTATCTGTTGGTGGACTTCACCAACATGGTGAGTGTCTTGAACGCATATTA
CGACATCAACAAAAAGTACAGGGCGATAAACTACCTTTTCGTGAATGCTGCGCAAGGTAT
CTTTGACGGTATAGATTGGATCGGAGCGGTCAAGGAGGTTTTCACCAATCCATTGGAGGC
AGTGACAAATCCGACATACAAGATACAACTGGTGGGCGTCAAGTCTAAAGATGACATGGG
GCTTATTTTCCAGGCCAATGTGTTTGGTCCGTACTACTTTATCAGTAAAATTCTGCCTCA
ATTGACCAGGGGAAAGGCTTATATTGTTTGGATTTCGAGTATTATGTCCGATCCTAAGTA
TCTTTCGTTGAACGATATTGAACTACTAAAGACAAATGCCTCTTATGAGGGCTCCAAGCG
TTTAGTTGATTTACTGCATTTGGCCACCTACAAAGACTTGAAAAAGCTGGGCATAAATCA
GTATGTAGTTCAACCGGGCATATTTACAAGCCATTCCTTCTCCGAATATTTGAATTTTTT
CACCTATTTCGGCATGCTATGCTTGTTCTATTTGGCCAGGCTGTTGGGGTCTCCATGGCA
CAATATTGATGGTTATAAAGCTGCCAATGCCCCAGTATACGTAACTAGATTGGCCAATCC
AAACTTTGAGAAACAAGACGTAAAATACGGTTCTGCTACCTCTAGGGATGGTATGCCATA
TATCAAGACGCAGGAAATAGACCCTACTGGAATGTCTGATGTCTTCGCTTATATACAGAA
GAAGAAACTGGAATGGGACGAGAAACTGAAAGATCAAATTGTTGAAACTAGAACCCCCAT
TTAATATATCTCTGCGTACATATGTATATATATATATGTGTGTATATACATGTATGTCTG
TATAGAAAACGCATATCAACTGATATATATACACGTGAAGCAAAAAAGGCATTGCCTCTG
TCAGAGAAACGAACTATCCTCGTTGCTATAGTGTCTTCTGCACTTATCGCGTAGACTTTC
TATTGGCACTCTGTCATGGCATTTAATCTTGTACACATTCTTTAATTCTTCTTGCAAAAG
AAGGTACTCTTCATGCAAATGATGACTTTGGAGTGAATTGACTAATCCTGAGTCGTTCCA
GAAA

>SACE0L03674g.cds
ATGAACAGGAAAGTAGCTATCGTAACGGGTACTAATAGTAATCTTGGTCTGAACATTGTG
TTCCGTCTGATTGAAACTGAGGACACCAATGTCAGATTGACCATTGTGGTGACTTCTAGA
ACGCTTCCTCGAGTGCAGGAGGTGATTAACCAGATTAAAGATTTTTACAACAAATCAGGC
CGTGTAGAGGATTTGGAAATAGACTTTGATTATCTGTTGGTGGACTTCACCAACATGGTG
AGTGTCTTGAACGCATATTACGACATCAACAAAAAGTACAGGGCGATAAACTACCTTTTC
GTGAATGCTGCGCAAGGTATCTTTGACGGTATAGATTGGATCGGAGCGGTCAAGGAGGTT
TTCACCAATCCATTGGAGGCAGTGACAAATCCGACATACAAGATACAACTGGTGGGCGTC
AAGTCTAAAGATGACATGGGGCTTATTTTCCAGGCCAATGTGTTTGGTCCGTACTACTTT
ATCAGTAAAATTCTGCCTCAATTGACCAGGGGAAAGGCTTATATTGTTTGGATTTCGAGT
ATTATGTCCGATCCTAAGTATCTTTCGTTGAACGATATTGAACTACTAAAGACAAATGCC
TCTTATGAGGGCTCCAAGCGTTTAGTTGATTTACTGCATTTGGCCACCTACAAAGACTTG
AAAAAGCTGGGCATAAATCAGTATGTAGTTCAACCGGGCATATTTACAAGCCATTCCTTC
TCCGAATATTTGAATTTTTTCACCTATTTCGGCATGCTATGCTTGTTCTATTTGGCCAGG
CTGTTGGGGTCTCCATGGCACAATATTGATGGTTATAAAGCTGCCAATGCCCCAGTATAC
GTAACTAGATTGGCCAATCCAAACTTTGAGAAACAAGACGTAAAATACGGTTCTGCTACC
TCTAGGGATGGTATGCCATATATCAAGACGCAGGAAATAGACCCTACTGGAATGTCTGAT
GTCTTCGCTTATATACAGAAGAAGAAACTGGAATGGGACGAGAAACTGAAAGATCAAATT
GTTGAAACTAGAACCCCCATTTAA

>SACE0L03674g.aa
MNRKVAIVTGTNSNLGLNIVFRLIETEDTNVRLTIVVTSRTLPRVQEVINQIKDFYNKSG
RVEDLEIDFDYLLVDFTNMVSVLNAYYDINKKYRAINYLFVNAAQGIFDGIDWIGAVKEV
FTNPLEAVTNPTYKIQLVGVKSKDDMGLIFQANVFGPYYFISKILPQLTRGKAYIVWISS
IMSDPKYLSLNDIELLKTNASYEGSKRLVDLLHLATYKDLKKLGINQYVVQPGIFTSHSF
SEYLNFFTYFGMLCLFYLARLLGSPWHNIDGYKAANAPVYVTRLANPNFEKQDVKYGSAT
SRDGMPYIKTQEIDPTGMSDVFAYIQKKKLEWDEKLKDQIVETRTPI*

Legend and notes

Lengths
The length, in codons, of coding sequences includes the stop codon, hence it is one unit longer than the protein length.

Genomic environment map
Click on the symbol of an element or a family to go to its corresponding page. Colors in the lane "protein encoding genes" indicate strandedness: shades of blue for direct orientation, shades of red for reverse orientation. Colors in the lane "protein family" are arbitrarly chosen in such a way that different protein families have different colors in the map.

Protein domain map
Domains are extracted from SwissProt files. Click on the symbol of a domain to extract the domain sequence.

Genemark image and list
Genemark computation of protein-coding potential of DNA was made from 1000 nucleotides upstream the open reading frame to 300 nucleotides downstream. Thus the open reading frame protein-coding potential appears on frame #3.

Sequences

Color	Nucleotide sequence and Coding sequence	Predicted translation product
RED	start and stop codons	Initial methionine and sequence end
BLUE	coding sequence	protein sequence
grey	non-coding sequence (upstream, downstream or intron)
grey	donor and acceptor splicing sites

Genetic Elements

SACE0L03674g

SACE0L03674g

Genomic environment map

Computed results

Homologs and Orthologs

Protein SACE0L03674p

Protein domain map

Computed results for SACE0L03674p

Gene Ontology terms

Sequence data

Nucleotide sequence

Coding sequence

Predicted translation product

Legend and notes