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BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= DEHA2D06468p (infer) YGL047W ALG13 Essential protein required
for the second step of dolichyl-linked oligosaccharide synthesis :
weakly similar to uniprot|P53178 Saccharomyces cerevisiae
[Debaryomyces hansenii CBS767]
         (212 letters)

Database: Genolevures3.aa 
           48,939 sequences; 23,992,848 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

|DEHA2D06468p (infer) YGL047W ALG13 Essential protein required fo...   431   e-121
|SACE0G04796p Catalytic component of UDP-GlcNAc transferase, requ...    95   5e-20
|ZYRO0G22352p (infer) YGL047W : similar to uniprot|P53178 Sacchar...    95   7e-20
|ERGO0G07436p Syntenic homolog of Saccharomyces cerevisiae YGL047...    86   2e-17
|SAKL0H23650p (infer) YGL047W : similar to uniprot|P53178 Sacchar...    84   9e-17
|KLLA0A04774p (infer) YGL047W : similar to uniprot|P53178 Sacchar...    84   1e-16
|CAGL0D06270p (infer) YGL047w : similar to uniprot|P53178 Sacchar...    80   1e-15
|KLTH0D03828p (infer) YGL047W : similar to uniprot|P53178 Sacchar...    77   1e-14
|YALI0E33275p (infer) YGL047w Hypothetical 22.7 kDa protein in SU...    76   3e-14

>|DEHA2D06468p (infer) YGL047W ALG13 Essential protein required for the second
           step of dolichyl-linked oligosaccharide synthesis :
           weakly similar to uniprot|P53178 Saccharomyces
           cerevisiae [Debaryomyces hansenii CBS767]
          Length = 212

 Score =   bits (1107), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 212/212 (100%), Positives = 212/212 (100%)

Query: 1   MTSVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFY 60
           MTSVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFY
Sbjct: 1   MTSVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFY 60

Query: 61  RQCVEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISESDVVI 120
           RQCVEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISESDVVI
Sbjct: 61  RQCVEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISESDVVI 120

Query: 121 SHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVKLGCCRKMTIEDMKSSQLKDC 180
           SHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVKLGCCRKMTIEDMKSSQLKDC
Sbjct: 121 SHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVKLGCCRKMTIEDMKSSQLKDC 180

Query: 181 ISEILSGPETFNKLPECSTTEVEGIIYHELVK 212
           ISEILSGPETFNKLPECSTTEVEGIIYHELVK
Sbjct: 181 ISEILSGPETFNKLPECSTTEVEGIIYHELVK 212


>|SACE0G04796p Catalytic component of UDP-GlcNAc transferase, required for the
           second step of dolichyl-linked oligosaccharide
           synthesis; anchored to the ER membrane via interaction
           with Alg14p; similar to bacterial and human
           glycosyltransferases [Saccharomyces cerevisiae]
          Length = 202

 Score = 95.1 bits (235), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 60/162 (37%), Positives = 94/162 (58%), Gaps = 14/162 (8%)

Query: 3   SVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYRQ 62
           ++  T GATV F +L+  + S +F  E +I  G  R+I+Q+G        ++ S EF   
Sbjct: 8   ALFVTCGATVPFPKLVSCVLSDEFCQE-LIQYGFVRLIIQFG--------RNYSSEFEHL 58

Query: 63  CVEDKELKQSLQLEVVS-GSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISE-SDVVI 120
             E    ++S ++ +   G  + +      + K +   ++ F FS  + S I + SD+VI
Sbjct: 59  VQERGGQRESQKIPIDQFGCGDTARQYVLMNGKLK---VIGFDFSTKMQSIIRDYSDLVI 115

Query: 121 SHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVKLG 162
           SHAGTGSI+D+LRL KPLIV  ND LM+ HQ+++AD+ V+LG
Sbjct: 116 SHAGTGSILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELG 157


>|ZYRO0G22352p (infer) YGL047W : similar to uniprot|P53178 Saccharomyces
           cerevisiae [Zygosaccharomyces rouxii]
          Length = 200

 Score = 94.7 bits (234), Expect = 7e-20,   Method: Compositional matrix adjust.
 Identities = 61/160 (38%), Positives = 88/160 (55%), Gaps = 14/160 (8%)

Query: 1   MTSVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFY 60
           + ++  T GATV F +L+E + S   IVE ++  G  R+IVQ+G        ++  E F 
Sbjct: 3   IKTIFVTCGATVPFPQLVEAVLS-PRIVEELLNLGFKRIIVQFG--------RNYRESFT 53

Query: 61  RQCVEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISE-SDVV 119
           +    D+ L  S   +   G   D  V  +        E++ F +S  I   I E +D+V
Sbjct: 54  QSINVDQSLPPS---QTYLGFNGDP-VHGFSQGSSGKLEIIGFEYSTRIQDVIQENADLV 109

Query: 120 ISHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELV 159
           ISHAGTGSI+D+LRL KPLIV  ND LM+ HQ+E+AD+  
Sbjct: 110 ISHAGTGSILDSLRLGKPLIVCVNDTLMDNHQQEIADQFA 149


>|ERGO0G07436p Syntenic homolog of Saccharomyces cerevisiae YGL047W [Eremothecium
           gossypii]
          Length = 203

 Score = 86.3 bits (212), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 54/161 (33%), Positives = 87/161 (54%), Gaps = 17/161 (10%)

Query: 3   SVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYRQ 62
           +V+ T GATV F  L+  +     + E +   G +R++VQYG        +  + EF RQ
Sbjct: 14  TVVVTCGATVPFPGLVNAVLDRRVLAE-LAQCGFSRVMVQYG--------RGFAAEFERQ 64

Query: 63  CVEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISESD-VVIS 121
                 ++ +   E + G   D++     + +++G E++ F F   + S I  S  +V+S
Sbjct: 65  VGAAGAVRAACDAEGLEGC--DAH-----AWRWQGLEIIGFAFHAQMESLIGTSAALVVS 117

Query: 122 HAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVKLG 162
           HAGTGSI+D LR +KPLIV  N+ L++ HQE++A     LG
Sbjct: 118 HAGTGSILDALRQQKPLIVCVNEALLDNHQEQIARRFEALG 158


>|SAKL0H23650p (infer) YGL047W : similar to uniprot|P53178 Saccharomyces
           cerevisiae [Lachancea kluyveri]
          Length = 198

 Score = 84.3 bits (207), Expect = 9e-17,   Method: Compositional matrix adjust.
 Identities = 58/157 (36%), Positives = 88/157 (56%), Gaps = 20/157 (12%)

Query: 3   SVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYR- 61
           ++L T GATV F  L++V+      ++ +      ++IVQ+G        K  S+EF R 
Sbjct: 6   NILVTCGATVPFPTLVKVLLQRQ-TLDHLKSMAFVKVIVQFG--------KGFSDEFERI 56

Query: 62  -QCVEDKELKQ-SLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFIS-ESDV 118
              + D E    S++LE +   +            Y+G E++   F+ +I + I   +DV
Sbjct: 57  LNGINDGEGGTISVELETLGTER-------VLHGWYKGVEIIGLEFTPNIEALIKLYADV 109

Query: 119 VISHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVA 155
           VISHAGTGSI+D+LRL+KPLI V ND LM+ HQE++A
Sbjct: 110 VISHAGTGSILDSLRLKKPLIAVVNDTLMDNHQEQIA 146


>|KLLA0A04774p (infer) YGL047W : similar to uniprot|P53178 Saccharomyces
           cerevisiae [Kluyveromyces lactis NRRL Y-1140]
          Length = 197

 Score = 83.6 bits (205), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 71/199 (35%), Positives = 105/199 (52%), Gaps = 25/199 (12%)

Query: 3   SVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYRQ 62
           +VL T GATV+F  L+E +     + E +   G  R+++QYG        +  S+ F  Q
Sbjct: 4   TVLVTCGATVSFPRLVETVLDRS-VTEKLKVLGYGRIVIQYG--------RGFSDTFL-Q 53

Query: 63  CVEDKEL-----KQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISES- 116
            VE K L     K+S  ++V+   +N       +     G E+  F FS+DI   I+ + 
Sbjct: 54  LVE-KHLGLFTEKKSCGIKVLDKIEN------LKVISVDGIEICGFEFSHDIEKLIANNI 106

Query: 117 DVVISHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVAD--ELVKLGCCRKMTIEDMKS 174
           D+VISHAGTGSI+D+LR+ K LIVV ND LM+ HQ+ +AD  E  KL        E++  
Sbjct: 107 DLVISHAGTGSILDSLRVGKKLIVVVNDTLMDNHQQLIADKFEQQKLLWSVHANTEELLR 166

Query: 175 SQLKDCISEILSGPETFNK 193
           +  +    E+L    T+NK
Sbjct: 167 ALDRSENEELLKIDNTYNK 185


>|CAGL0D06270p (infer) YGL047w : similar to uniprot|P53178 Saccharomyces
           cerevisiae [Candida glabrata CBS 138]
          Length = 198

 Score = 80.1 bits (196), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 59/161 (36%), Positives = 83/161 (51%), Gaps = 10/161 (6%)

Query: 3   SVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYRQ 62
           S   T GATV F  L+E + + +F V  +   G   + VQ+G   +   Q   S    R 
Sbjct: 2   SAFVTCGATVPFPALVEAVLAPEF-VGCLSREGYRVLCVQFGRGYDFEAQ-FTSVTCTRM 59

Query: 63  CVEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISE-SDVVIS 121
            +E  E+ +  QL            VT    K +   ++ F +SN+I   I    DVVIS
Sbjct: 60  PLESAEVSELRQLV-------RDERVTVMGYKVQDVVVLGFAYSNNILQIIDRYGDVVIS 112

Query: 122 HAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVKLG 162
           HAGTGSI+D+LRL K LIVV N  LM+ HQ+++A++   LG
Sbjct: 113 HAGTGSILDSLRLNKKLIVVVNHTLMDNHQKQIAEKFQNLG 153


>|KLTH0D03828p (infer) YGL047W : similar to uniprot|P53178 Saccharomyces
           cerevisiae [Kluyveromyces thermotolerans]
          Length = 198

 Score = 77.0 bits (188), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 53/156 (33%), Positives = 84/156 (53%), Gaps = 14/156 (8%)

Query: 2   TSVLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYR 61
           ++V+ T GATV F ELI  +     +++T++    TR+IVQYG    +   + +      
Sbjct: 3   STVVVTCGATVPFPELIAALLDKQ-VLDTLLRMHYTRLIVQYGRGYTSKFAQLLG----- 56

Query: 62  QCVEDKELKQSLQLEVVSGSQN-DSNVVTYRSNKYRGFEMVVFPFSNDIGSFISE-SDVV 119
            CV     +  ++    +G+ N  + V       Y+  E+  F F+  I   + + +D+V
Sbjct: 57  -CV-----RAHVEEPADNGASNLAAGVEVQVRGHYQSLEVCGFEFTGAIHELLRDNADLV 110

Query: 120 ISHAGTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVA 155
           ISHAGTGSI+D LRL K L+VV N  LM+ HQ ++A
Sbjct: 111 ISHAGTGSIVDALRLGKRLLVVANTSLMDNHQLQIA 146


>|YALI0E33275p (infer) YGL047w Hypothetical 22.7 kDa protein in SUG1-RNA15
           intergenic region : similar to uniprot|P53178
           Saccharomyces cerevisiae [Yarrowia lipolytica CLIB122]
          Length = 196

 Score = 75.9 bits (185), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 50/157 (31%), Positives = 81/157 (51%), Gaps = 39/157 (24%)

Query: 4   VLFTSGATVTFRELIEVITSYDFIVETIIGNGITRMIVQYGNEIETGTQKHVSEEFYRQC 63
           VL T+G TV F  LIE++ S++ I  T+   G ++M VQYG        +H+  + +++ 
Sbjct: 30  VLVTTGGTVPFEALIELVLSHESIT-TLSQLGFSKMRVQYGR-----GNRHIFTKHHKEG 83

Query: 64  VEDKELKQSLQLEVVSGSQNDSNVVTYRSNKYRGFEMVVFPFSNDIGSFISESDVVISHA 123
           V             ++G                      F +++D+   +S + +VISHA
Sbjct: 84  VMS-----------ITG----------------------FEYTDDLAGEMSRAHLVISHA 110

Query: 124 GTGSIIDTLRLEKPLIVVTNDKLMNKHQEEVADELVK 160
           GTGS++D LR+ K  +VV N KLM+ HQ E+A+EL +
Sbjct: 111 GTGSVLDALRIGKHPVVVVNSKLMDNHQIEIAEELFR 147


BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.